Immunohistochemical analysis of paraffin-embedded human testis using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human spleen using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

A431 cells were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

HeLa nuclear fraction and cytoplasmic fraction were subjected to SDS PAGE saparately, followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped, reblotted with lamin B1 (12987-1-AP) & GAPDH (60004-1-Ig) antibodies as the loading control.

IP result of anti-NF-κB p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were stained with 0.2ug p65; RELA antibody (10745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IP result of anti-NFKB1,p105,p50 (IP:14220-1-AP, 4ug; Detection:14220-1-AP 1:3000) with Jurkat cells lysate 2280 ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NFKB1 antibody (14220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (14220-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

G66920-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G66920-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

IF result of the antibody CUX1 (11733-1-AP, 1:300) with mouse cortex, Data from Dr. Lee O. Vaasjo Muñoz, University of Chicago. .

Various lysates were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-CUX1 (IP:11733-1-AP, 4ug; Detection:11733-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

PC-3 cells were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

WB result of VPS34 (C terminal) antibody (12452-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 (C terminal) transfected Jurkat cells.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using VPS34 (C terminal) antibody (12452-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

WB result of 10409-2-AP (NFKB2 antibody).

IP result of anti-NFKB2 (IP:10409-2-AP, 4ug; Detection:10409-2-AP 1:600) with K-562 cells lysate 1200ug.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10409-2-AP (NFKB2 Antibody) at dilution of 1:50 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10409-2-AP (NFKB2 Antibody) at dilution of 1:50 (under 40x lens).

Immunofluorescent analysis of HeLa cells using 10409-2-AP (NFKB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

A431 cells were subjected to SDS PAGE followed by western blot with 15506-1-AP (NFKB1,p105,p50-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.